Key Points:
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Intended Use (IN VITRO DIAGNOSTIC USE)
PCRopsis™ Reagent SRVD is intended for extraction-free amplification of RNA or DNA from properly collected and transported saliva samples.
- Reagent SRVD may replace extraction procedures for many applications (e.g., life science research, veterinary pathogen research, etc.), but it must be verified by the end user.
PCRopsis™ Reagent SRVD is an alternative for:
- Thermo Fisher Scientific: various MagMAX kits
- Qiagen: RNeasy Saliva Kit, DNeasy Blood & Tissue Kit, etc.
- Numerous other silica- or bead-based RNA and DNA extraction kits
The Science Behind Reagent SRVD
PCRopsis™ Reagent SRVD is engineered to simultaneously bind a variety of RT-qPCR / PCR inhibitors found in saliva specimens, lyse microorganisms and stabilize RNA and DNA in a manner that's compatible with RT-qPCR / PCR. The product consist of a proprietary mixture of peptides, salts, stabilizers, RVD Enhancer, buffers and sodium azide to achieve these tasks. Reagent SRVD allows for extraction-free amplification of specimen RNA / DNA without performing nucleic acid isolation, centrifugations or other sample manipulations which may introduce errors, contaminants and/or skew the representation of RNA fragments.
User should verify the applicability of Reagent SRVD for unintended applications.
User should verify the applicability of Reagent SRVD for unintended applications.
Traditional RNA Extraction Results in Significantly Different Levels of miRNA ... this bias can affect results.
Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal
Rikki A. M. Brown, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Kirsty L. Richardson & Peter J. Leedman BMC Biotechnology volume 18, Article number: 16 (2018) - Link to manuscript
A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
Ryan K.Y. Wong, Meabh MacMahon, Jayne V. Woodside & David A. Simpson BMC Genomics volume 20, Article number: 446 (2019) - Link to manuscript
RNA Preservation Agents and Nucleic Acid Extraction Method Bias Perceived Bacterial Community Composition
Ana McCarthy, Edna Chiang, Marian L. Schmidt, Vincent J. Denef, PLOS ONE (2015) - Link to manuscript
Rikki A. M. Brown, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Kirsty L. Richardson & Peter J. Leedman BMC Biotechnology volume 18, Article number: 16 (2018) - Link to manuscript
A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
Ryan K.Y. Wong, Meabh MacMahon, Jayne V. Woodside & David A. Simpson BMC Genomics volume 20, Article number: 446 (2019) - Link to manuscript
RNA Preservation Agents and Nucleic Acid Extraction Method Bias Perceived Bacterial Community Composition
Ana McCarthy, Edna Chiang, Marian L. Schmidt, Vincent J. Denef, PLOS ONE (2015) - Link to manuscript
Reagent SRVD does not use "capture & release" RNA / DNA technology (e.g., columns, beads, etc.), therefore it does not exhibit this bias.
Studies with PCRopsis™ Reagent SRVD
Reagent SRVD has a Limit of Detection Less than 3.9 viral copies / uL in 100% Saliva
Human saliva was spiked with inactivated SARS-CoV-2 (ATCC) and processed with Reagent SRVD. Five viral gene sites were amplified through RT-qPCR, in duplicates. Reagent SRVD mediated amplification of tested gene regions with Ct values between 33 ~38 at 3.9 viral copies / uL, suggesting even lower viral copies may be detectable.
RT-qPCR was performed using IDT qPCR probe assay and Promega GoTaq® Probe 1-Step RT-qPCR System. |
Satisfaction Guarantee - Warranty Summary
Entopsis guarantees the performance of Reagent SRVD to facilitate the direct amplification of coronaviruses using RT-qPCR from saliva and the sample is properly stored. Within 7 days of purchase, contact Entopsis (info@entopsis.com) should Reagent SRVD fail to perform as described above or in the Certificate of Analysis (CoA). In such a scenario, Entopsis will re-test the Lot in question using remaining reagent at our facility at our expense (purchaser does not need to ship product to Entopsis). If the re-tested Lot fails to meet our performance criteria, Entopsis will replace the faulty reagent free of charge. If the Lot meets our performance criteria as stated on the CoA for the Lot in question, then no additional action will be taken.
Please keep in mind that purchasers may use Reagent SRVD for numerous applications, sample types, gene targets, conditions, etc. that are untested and thereby may not work as desired. The purchaser must determine the suitability of the Reagent SRVD for its particular use. However, the Entopsis scientific team can assist in the optimization of the product for your desired application. Refunds will not be provided due to a lack of product compatibility with purchaser's desired application. This is why small test volumes of Reagent SRVD are offered.
THIS WARRANTY ONLY APPLIES IF PRODUCTS ARE PURCHASED FROM AN APPROVED VENDOR (E.G., ENTOPSIS).
Complete Terms and Conditions
Please keep in mind that purchasers may use Reagent SRVD for numerous applications, sample types, gene targets, conditions, etc. that are untested and thereby may not work as desired. The purchaser must determine the suitability of the Reagent SRVD for its particular use. However, the Entopsis scientific team can assist in the optimization of the product for your desired application. Refunds will not be provided due to a lack of product compatibility with purchaser's desired application. This is why small test volumes of Reagent SRVD are offered.
THIS WARRANTY ONLY APPLIES IF PRODUCTS ARE PURCHASED FROM AN APPROVED VENDOR (E.G., ENTOPSIS).
Complete Terms and Conditions
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