Short answer... yes, for E. coli and S. aureus. Other bacteria are being tested.
There is great value in detecting microorganisms and tumor cells in urine and blood samples. To date, DNA from cells in samples has been isolated and then used in PCR for detection of relevant DNA fragments. However, the process of isolating DNA results in the lost of some DNA and involves a number of steps, opening the possibility for error and contamination. Alternatively, cells within samples can be lysed at 95°C ~ 100°C and the released DNA directly used for PCR, without any lost of DNA in a simple, mostly error-free fashion. However, lysed cells release molecules that interfere with PCR and potentially leading to a lack of DNA amplification.
To answer the question posed in the title, our team compared DNA recovered from thermally lysed cells in urine or plasma and processed using PCRopsis tubes to DNA recovered from the same samples using Qiagen's QIAamp® DNA Kit. We used PCRopsis 5x PCR Master Mix to ensure we obtain optimal PCR amplification. The relevant results and statistical analysis are in the link below. In summary, one can detect DNA fragments from thermally lysed cells within urine or plasma that were processed with PCRopsis tubes to a level that's statistically comparable to Qiagen's QIAamp® DNA Kit.
The PCRopsis tubes offer the following advantages over QIAamp® DNA Kit:
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