Protocol

Concentration of RNA, DNA, proteins and cells from aqueous samples without any equipment

PCRopsis Concentrator tubes come pre-loaded with a sterile, proprietary substrate.
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1. Add 0.5 ~ 2 mL of sample to be concentrated / 2 mL PCRopsis Concentrator tube
   1. TIP: thoroughly mix sample with PCRopsis Concentrator dried substrates for best results
2. Let sample incubate at room temperature for 2 ~ 20 minutes depending on your desired level of sample concentration
3. Remove concentrated supernatant into a fresh tube by sliding the pipette tip along the edge of the tube to the bottom of the tube
4. Use concentrated supernatant in desired applications

PCRopsis Concentrator is an Alternative to:

1. RNA / DNA isolation approaches aimed at concentrating RNA / DNA
2. Protein concentration using filters
3. Concentration of cells through centrifugation

​Performance

The PCRopsis Concentrator consistently removes water from samples without centrifugation at room temperature. Each lots is carefully sterilized and tested to ensure maximum quality.

​Principal

The PCRopsis Concentrator contains a proprietary substrate engineered to quickly and selectively absorb water with minimal absorption of RNA, DNA, proteins or cells. This approach allows you to concentrate RNA, DNA, proteins and cells from samples without utilizing time-consuming, expensive and labor intensive approaches. As such, the PCRopsis Concentrator offers an equipment-free solution that's well suited for high throughput applications and automation.

Decrease in Volume of a 1 mL Sample Over 15 minutes

• Sample: ​
  • 1:1 mixture of human plasma with PBS
• Absorption Dynamics:
  • Fairly linear absorption of water from sample between 0~6 minutes
  • ~500 μL of water from the samples is absorbed within 5 minutes with rocking
  • ~500 μL of water from the samples is absorbed within 8 minutes without rocking (stationary)
  • 700~750 μL of water from the samples is absorbed at 15 minutes
• NOTE: Customers should determine the optimal incubation time with PCRopsis Concentrators using their own samples since absorption dynamics are specific to each sample types

​Applications

Concentration of RNA, DNA, proteins and cells
High throughput automation
​Cellular assays
Studies in low-resource settings
Banking WBC, PBMC​ or specific cell types
​Life science research
Routine applied testing

Studies with PCRopsis Concentrator

(Click on the icons below for more details) 

PCRopsis Concentrator Concentrates DNA in Plasma

PCRopsis Concentrator Concentrates Proteins from Plasma

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